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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/797

Title: Ascomycetous yeast species recovered from grapes damaged by honeydew and sour rot
Authors: Malfeito-Ferreira, Manuel
Loureiro, Virgilio
Barata, Andre
Seborro, F.
Belloch, C.
Keywords: damaged grapes
selective culture media
sour rot
spoilage yeasts
zygoascus hellenicus
zygosaccharomyces bailii
zygosaccharomyces bisporus
Issue Date: 2007
Publisher: Society of Applied Microbiology
Citation: "Journal of Applied Microbiology". ISSN 1364-5072. 104 (2008) 1182-1191
Abstract: Aims: To identify ascomycetous yeasts recovered from sound and damaged grapes by the presence of honeydew or sour rot. Methods and Results: In sound grapes, the mean yeast counts ranged from 3.20 ± 1.04 log CFU g-1 to 5.87 ± 0.64 log CFU g-1. In honeydew grapes, the mean counts ranged from 3.88 ± 0.80 log CFU g-1 to 6.64 ± 0.77 log CFU g-1. In sour rot grapes counts varied between 6.34 ± 1.03 and 7.68 ± 0.38 log CFU g-1. Hanseniaspora uvarum was the most frequent species from sound samples. In both types of damage, the most frequent species were Candida vanderwaltii, H. uvarum and Zygoascus hellenicus. The latter species was recovered in high frequency because of the utilization of the selective medium DBDM (Dekkera ⁄ Brettanomyces differential medium). The scarce isolation frequency of the wine spoilage species Zygosaccharomyces bailii (in sour rotten grapes) and Zygosaccharomyces bisporus (in honeydew affected grapes) could only be demonstrated by the use of the selective medium ZDM (Zygosaccharomyces differential medium). Conclusions: The isolation of several species only from damaged grapes indicates that damage constituted the main factor determining yeast diversity. The utilization of selective media is required for eliciting the recovery of potentially wine spoilage species. Significance and Impact of the Study: The impact of damaged grapes in the yeast ecology of grapes has been underestimated
URI: http://hdl.handle.net/10400.5/797
ISSN: 1364-5072
Appears in Collections:DBEB - Artigos de Revistas

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