Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/7072
Título: Detailed dimethylacetal and fatty acid composition of rumen content from lambs fed lucerne or concentrate supplemented with soybean oil
Autor: Alves, Susana P.
Silva, José Santos
Cabrita, Ana R. J.
Fonseca, António J. M.
Bessa, Rui J. B.
Palavras-chave: Alfafa
Fatty acids
Thin-layer chromatographie
Data: 4-Mar-2013
Editora: Wolf-Hagen Schunck, Max Delbrueck Center for Molecular Medicine, Germany
Citação: Alves, S.P., Silva, J.S., Cabrita, A.R.J., Fonseca, A.J.M., Bessa, R.J.B. (2013). Detailed dimethylacetal and fatty acid composition of rumen content from lambs fed lucerne or concentrate supplemented with soybean oil. PLoS ONE 8(3), e58386, 1-14. doi:10.1371/journal.pone.0058386
Resumo: Lipid metabolism in the rumen is responsible for the complex fatty acid profile of rumen outflow compared with the dietary fatty acid composition, contributing to the lipid profile of ruminant products. A method for the detailed dimethylacetal and fatty acid analysis of rumen contents was developed and applied to rumen content collected from lambs fed lucerne or concentrate based diets supplemented with soybean oil. The methodological approach developed consisted on a basic/ acid direct transesterification followed by thin-layer chromatography to isolate fatty acid methyl esters from dimethylacetal, oxo- fatty acid and fatty acid dimethylesters. The dimethylacetal composition was quite similar to the fatty acid composition, presenting even-, odd- and branched-chain structures. Total and individual odd- and branched-chain dimethylacetals were mostly affected by basal diet. The presence of 18:1 dimethylacetals indicates that biohydrogenation intermediates might be incorporated in structural microbial lipids. Moreover, medium-chain fatty acid dimethylesters were identified for the first time in the rumen content despite their concentration being relatively low. The fatty acids containing 18 carbon-chain lengths comprise the majority of the fatty acids present in the rumen content, most of them being biohydrogenation intermediates of 18:2n26 and 18:3n23. Additionally, three oxo- fatty acids were identified in rumen samples, and 16-O-18:0 might be produced during biohydrogenation of the 18:3n23.
Descrição: Articles in International Journals
Peer review: yes
URI: http://hdl.handle.net/10400.5/7072
DOI: 10.1371/journal.pone.0058386
ISSN: 1932-6203
Aparece nas colecções:DPASA - Artigos de revista
CIISA - Artigos em revistas internacionais

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