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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/578

Title: Obtenção in vitro do teleomorfo de Cylindrocarpon destructans, Neonectria Radicicola
Authors: Oliveira, Helena
Rego, Cecilia
Cabral, A.
Nascimento, Teresa
Keywords: cylindrocarpon destructans
neonectria radicicola
pé-negro da videira
black-foot of grapevine
Issue Date: May-2003
Publisher: ESA
Citation: Encontro Nacional de Protecção Integrada, 6º, Castelo Branco, 2003
Abstract: Black-foot is an important grapevine disease, caused by the fungus Cylindrocarpon destructans, which causes a darkening of the basal-end of the plant, leading to early decline and death of young grapevines. Both, C. destructans and C. obtusisporum are considered responsible for this disease, although only the former was isolated in Portugal. Neonectria radicicola (teleomorph of C. destructans) is an Ascomycota forming perithecia on host tissue, but such structures haven’t been observed yet in the country in grapevine. There is some uncertainty concerning the sexual behaviour of the fungus, some authors considering this as a homothallic species while other considered it as heterothallic. Attempting to obtain the teleomorph of C. destructans, a collection of 15 isolates was used, originated from different regions of the country. Crosses between isolates (120 pairs) were done in Petri dishes containing Carnation Leaf Agar (CLA), under Near-UV light (NUV) at 6ºC. For some crosses, perithecia formation began after three months of incubation. After five months of incubation, morphological and biometrical traits of perithecia, asci and ascospores were evaluated. The teleomorph was only obtained when crossing distinct isolates, suggesting a tendency for an heterothallic behaviour of the species. Four isolates can be pointed out as more fertile (Cy68, Cy36, Cy76, Cy111), with Cy68 forming perithecia in 60% of crossings involving it, while the remaining produced perithecia in 33% of crosses. The identification of these isolates will allow enlarging the scope of the study to all C. destructans isolates under collection, aiming to confirm the sexual behaviour of this species.
URI: http://hdl.handle.net/10400.5/578
Appears in Collections:SSPV - Comunicações em Actas de Conferências

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