Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/5192
Título: New insights in Ilyonectria black foot disease of grapevine
Outros títulos: Novas perspectivas sobre o pé negro da videira causado por Ilyonectria
Autor: Cabral, Ana Cristina Garcia Pereira
Orientador: Oliveira, Maria Helena Mendes da Costa Ferreira Correia de
Crous, Peter Willem
Rego, Maria Cecília Farinha
Palavras-chave: grapevine
black foot disease
mating type genes
protoplast transformation
soilborne inoculum
quantitative PCR
Data de Defesa: 2012
Editora: ISA/UTL
Citação: Cabral, A.C.G.P. - New insights in Ilyonectria black foot disease of grapevine. Lisboa: ISA, 2012
Resumo: Considering the growing importance of black foot disease of grapevine, this study was aimed to deeply understand details on taxonomy, genetics, biology and pathological behaviour of its main causal agents, previously attributed mostly to Ilyonectria liriodendri and I. macrodidyma. A multi-gene analysis of a collection of Ilyonectria isolates, along with morphological characterisation, enabled the description of 12 species from I. radicicola and four from I. macrodidyma complexes. Among these, pathogenicity experiments revealed I. lusitanica, I. estremocensis and I. europaea as more virulent to grapevine than I. liriodendri and I. macrodidyma. The entire mating-type loci of I. liriodendri and of species from the I. macrodidyma complex were obtained. While the idiomorph structure of species from the latter matches that of other heterothallic Hypocreales, the organization of the mating-type loci in I. liriodendri seems unique, suggesting a potential pseudo-heterothallism. Soilborne inoculum is accepted to contribute significantly to initiate black foot disease in grapevine plants. qPCR amplification from DNA soil samples demonstrate that rotation can reduce the levels of Ilyonectria in nurseries, and that levels of infestation in vineyard soils are lower than in nursery or mother-plant soils. Additionally, a protoplast transformation protocol is presented for the stable integration of the GFP gene in the genome of I. liriondendri, enabling future downstream functional genetic studies.
Descrição: Doutoramento em Engenharia Agronómica - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/5192
Aparece nas colecções:BISA - Teses de Doutoramento / Doctoral Thesis

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