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|Title: ||Development and use of a new medium to detect yeasts of the genera Dekkera/Brettanomyces|
|Authors: ||Malfeito-Ferreira, Manuel|
|Issue Date: ||2001|
|Publisher: ||The Society for Applied Microbiology|
|Citation: ||"Journal of Applied Microbiology". ISSN 1364-5072. 90 (2001) 1-12|
|Abstract: ||Aims: The objectives of this work were to develop a selective and/or differential medium able
to effciently recover Dekkera/Brettanomyces sp. from wine-related environments and to
determine the relationship between these yeasts and the 4-ethylphenol content in a wide range
Methods and Results: The selectivity of the developed medium was provided by the addition
of ethanol, as single carbon source, and cycloheximide. The inclusion of bromocresol green
evidenced acid-producing strains. The inclusion of p-coumaric acid, substrate for the
production of 4-ethylphenol, enabled the differentiation by smell of Dekkera/Brettanomyces sp.
from all other yeast species growing in the medium. The medium was used either by plating
after membrane filtration or by the Most Probable Number (MPN) technique. In 29 white and
88 red randomly collected wines, these yeasts were found only in red wines at levels up to
2500 MPN ml±1, but constituted less than 1% of the total microbial flora. In red wines, 84%
showed detectable amounts of 4-ethylphenol up to 4430 lg l±1 while 28% of the white wines
showed detectable levels up to 403 lg l±1.
Conclusions: The use of the medium proposed in this work evidenced the presence of low
relative populations of Dekkera/Brettanomyces sp. even in wines contaminated by fast-growing
yeasts and moulds.
Significance and Impact of the Study: Further ecological studies on Dekkera/Brettanomyces
sp. should take into account the use of highly specific culture media in order to establish
their true occurrence in nature|
|Appears in Collections:||DBEB - Artigos de Revistas|
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