UTL Repository >
ISA - Instituto Superior de Agronomia >
DBEB - Departamento de Botânica e Engenharia Biologica >
DBEB - Artigos de Revistas >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/490

Title: Identification of yeasts isolated from wine-related environments and capable of producing 4-ethylphenol
Authors: Malfeito-Ferreira, Manuel
Loureiro, Virgilio
Dias, L.
Dias, S.
Sancho, T.
Stender, H.
Querol, A.
Keywords: 4-ethylphenol
yeast
wine
Issue Date: 2002
Publisher: Elsevier
Citation: "Food Microbiology". ISSN 0740-0020. Article in Press
Abstract: The ability to produce 4-ethylphenol from the substrate p-coumaric acid in synthetic media was evaluated for several yeast species associated with wine production.Molar conversion rates as high as 90% were found by only Dekkera bruxellensis, D. anomala and by some unidentified strains isolated from wine-related environments.Other unidentified strains produced traces of 4-ethylphenol. All unidentified strains showed the same cultural characteristics as D. bruxellensis when grown on DBDM (Dekkera/Brettanomyces differential medium) agar.The determination of long-chain fatty acid compositions and the utilization of peptide nucleic acid (PNA) probes specific for D. bruxellensis showed that the unidentified strains did not belong to this species.Further identification, by restriction pattern generated from PCR-amplification of the 5.8S rRNA gene and the two internal transcribed spacers (ITS), assigned the unidentified strains to Candida cantarelli, C. wickerhamii, Debaryomyces hansenii, Kluyveromyces lactis and Pichia guilliermondii.However, only some strains of P. guilliermondii were capable of converting p-coumaric acid into 4-ethylphenol with efficiencies close to those observed in D. bruxellensis and D. anomala.
Description: www.elsevier.nl/locate/jnlabr/yfmic <p> DOI:10.1016/S0740-0020(02)00152-1
URI: http://hdl.handle.net/10400.5/490
ISSN: 0740-0020
Appears in Collections:DBEB - Artigos de Revistas

Files in This Item:

File Description SizeFormat
REPOS-DekkeraPichia.pdf192.67 kBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 
Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE