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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/4455

Title: Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
Authors: Lourenço, António
Machado, Henrique
Brito, Luísa
Keywords: biofilms
Calgary biofilm device
Listeria monocytogenes
low temperature
sanitizers
Issue Date: Mar-2011
Publisher: Wiley-Blackwell
Citation: "Journal of Food Science". ISSN 1750-3841. 76(2) (2011) M143-M148
Abstract: The biofilm-forming ability of 21 Listeria monocytogenes isolates, previously pulsotyped and corresponding to 16 strains, from different origins was evaluated using the Calgary Biofilm DeviceR , at 37 ◦C. Biofilms of 4 selected strains were also produced either on pure cultures or on co-cultures with Pseudomonas aeruginosa (PAO1), at 12 ◦C and at 37 ◦C. For these biofilms, the minimum biofilm eradication concentrations (MBECs) of 4 commercial dairy sanitizers (1 alkyl amine acetate based—T99, 2 chlorine based—T66 and DD, and 1 phosphoric acid based—BP) were determined. Listeria monocytogenes biofilms grown, either at 37 ◦C or 12 ◦C, were able to achieve similar cell densities by using different incubation periods (24 h and 7 d, respectively). In co-culture biofilms, P. aeruginosa was the dominant species, either at 37 ◦C or at 12 ◦C, representing 99% of a total biofilm population of 6 to 7 log CFU/peg. Co-culture biofilms were generally less susceptible than L. monocytogenes pure cultures. More interestingly, the biofilms produced at 12 ◦C were usually less susceptible to the sanitizers than when produced at 37 ◦C. Single or co-culture biofilms of L. monocytogenes and PAO1, particularly produced at 12 ◦C, retrieved MBEC values for agents T99 and BP that were, at times, above the maximum in-use recommended concentrations for these agents. The results presented here reinforce the importance of the temperature used for biofilm formation, when susceptibility to sanitizers is being assessed
Description: Available at B-on
Peer Reviewed: yes
URI: http://hdl.handle.net/10400.5/4455
ISSN: 1750-3841
Publisher version: DOI 10.1111.j.1750-3841.2010.02011.x
Appears in Collections:CBAA - Artigos de Revista

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