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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/4254

Title: Kinetics of production and characterization of the fucose-containing exopolysaccharide from Enterobacter A47
Authors: Torres, Cristiana A.V.
Marques, Rodolfo
Antunes, Sílvia
Alves, Vitor D.
Sousa, Isabel
Ramos, Ana Maria
Oliveira, Rui
Freitas, Filomena
Reis, Maria A.M.
Keywords: Enterobacter
glycerol byproduct
Issue Date: 2011
Publisher: Elsevier
Citation: "Journal of Biotechnology". ISSN 0168-1656. 156 (2011) 261-267
Abstract: A fucose-containing exopolysaccharide (EPS) was produced by the bacterium Enterobacter A47 using glycerol byproduct from the biodiesel industry. The analysis of kinetic data suggested a partially growth associated EPS synthesis model. Although the EPS was composed of fucose, galactose and glucose at all cultivation stages, their relative proportion has varied considerably during the run. At the beginning (24 h), glucose was the main component (82.4 wt.%), being fucose and galactose minor components (5.0 wt.% and 10.9 wt.%, respectively), while at the end (96 h) it was composed of 26.0 wt.% fucose, 28.9 wt.% galactose and 43.7 wt.% glucose. The acyl groups content and composition have also changed, reaching their maximum content (19.2 wt.%) at the end of the run. Moreover, the molecular weight has increased linearly during the run (from 8 × 105 to 5 × 106). The changes observed in EPS composition and molecular weight have also had an impact upon the polymer’s intrinsic viscosity, as shown by its linear increase from 3.95 to 10.72 dL g−1. The results suggest that the culture might have synthesized at least two distinct EPS, with different sugar composition and average molecular weight, which predominated at different cultivation stages
Description: Available at ScienceDirect
Peer Reviewed: yes
URI: http://hdl.handle.net/10400.5/4254
ISSN: 0168-1656
Publisher version: www.elsevier.com/locate/jbiotec
Appears in Collections:DAIAT - Artigos de Revistas

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