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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/4188

Title: Identificação e sequenciação de genes da via da lenhificação em bibliotecas BAC de Eucalyptus globulus
Authors: Barrela, Ricardo Joel Martins Bernardino Marques
Advisor: Paiva, Jorge Caldeira Pinto
Almeida, Maria Helena Ribeiro
Keywords: Eucalyptus globulus
BAC library
lignin biosynthesis pathway
3 D pool
Issue Date: 2011
Publisher: ISA/UTL
Citation: Barrela, Ricardo Joel Martins Bernardino Marques - Identificação e sequenciação de genes da via da lenhificação em bibliotecas BAC de Eucalyptus globulus. Lisboa: ISA, 2011
Abstract: In this work we characterized two libraries containing BAC clones - EGC_Ba and EGC_Bb - obtained from fragments of genomic DNA of Eucalyptus globulus digested with HindIII and EcoRI respectively. The estimated genome coverage for the two libraries was 16X. Both libraries had inserts with average size of 115kb. Assessment of contamination by extranuclear genome was performed by hybridization of high-density colony filters, ensuring a low rate of contamination. The probability associated with the attempt to find specific sequences in the libraries was calculated at a value greater than 0.99. The identification of several genes involved in lignin biosynthetic pathway, transcription factors and peripheral regions of interest via PCR was performed using an innovative scheme of three-dimensional pools. Of the clones identified, thirteen were chosen for sequencing. The analysis of the sequences obtained allowed identifying the reduced presence of repetitive elements and microsatellites. The identified clones can be used in future researches involving the isolation of genes of QTL and genomic sequence comparison with other species of Eucalyptus.
Description: Mestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/4188
Appears in Collections:BISA - Dissertações de Mestrado / Master Thesis

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