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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/4164

Title: Queijo de coalho do sertão Alagoano: enterotoxigenicidade de S. aureus pela reacção em cadeia polimerase (PCR)
Authors: Fonte, Ângela Isabel Espinha da
Advisor: Brito, Maria Luísa de Castro e
Delgado, Maria Cristina
Keywords: coalho cheese
food poisoning
Staphylococcus aureus
staphylococcal enterotoxins
PCR
sertão Alagoano
Issue Date: 2011
Publisher: ISA/UTL
Citation: Fonte, Ângela Isabel Espinha da - Queijo de coalho do sertão Alagoano: enterotoxigenicidade de S.aureus pela reacção em cadeia da polimerase (PCR). Lisboa: ISA, 2011
Abstract: Staphylococcal enterotoxins (SE) are emetic toxins and are one of the main causes of food poisoning in humans. It is known that about 95% of staphylococcal food poisoning outbreaks are caused by SE types SEA to SEE. In this study, to investigate the distribution of staphylococcal enterotoxin genes (sea to see) in Staphylococcus aureus, 40 isolates obtained in Alagoas-Brazil from “coalho” cheese were analyzed by PCR. All S. aureus isolates were found to be positive for one or more se genes. Among the 40 isolates of S. aureus positive by PCR, the genotypes found were sea (32.5%), seb(22.5%), sec (42.5%), sed (25%), see (42.5%) and associations sea+sec (15%) and sec+see (30%). During this work, in seven samples of “coalho” cheese, obtained from four different industrial brands of sertão Alagoano the occurrence of S. aureus, Salmonella spp., Listeria and microorganisms indicators of fecal contamination was also determined. All of the samples were positive to coagulase positive staphylococci, and three samples (42.9%) were positive for Salmonella spp.. Regarding the contamination by total coliforms, all samples showed values above the limits established in the Brazilian legislation and 57.1% presented fecal coliforms. Such values are alarming since the majority of the analyzed products were inappropriate for consumption.
Description: Mestrado em Engenharia Alimentar - Biotecnologia Microbiana - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/4164
Appears in Collections:BISA - Dissertações de Mestrado / Master Thesis

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