Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/4114
Título: Detecção de fungos do género Ilyonectria em viveiro vitícola
Autor: Cardoso, Margarida do Nascimento
Orientador: Oliveira, Maria Helena Mendes da Costa Ferreira Correia de
Palavras-chave: black foot
Ilyonectria spp
Cylindrocarpon spp
multiplex nested-PCR
microbiological isolation
Data de Defesa: 2011
Editora: ISA/UTL
Citação: Cardoso, Margarida do Nascimento - Detecção de fungos do género Ilionectria em viveiro vitícola. Lisboa: ISA, 2011
Resumo: Black foot of grapevine is an important disease caused primarily by fungi of the genus Ilyonectria. These pathogens affect grapevine nurseries and young vineyards, causing the decline and death of plants. In the nursery, the infections are mainly attributed to infected propagation material and soilborne inoculum, thus hampering the selection of healthy plant material. The aim of this study was to detect/identify possible sources of inoculum for Ilyonectria spp. and "Cylindrocarpon" pauciseptatum, also associated with the disease, throughout the different nursery stages. Samples of plant material (rootstock and scion mother plants, grafted-cuttings and rooted graftlings), soil (from mother-fields, nurseries and plots in rotation), callusing-medium (before and after utilization), air chambers and warehouses, water from hydration tanks and tap water, cutting tools and paraffin, were analysed by classical and molecular techniques (nested-PCR and multiplex nested-PCR). Multiplex nested-PCR revealed to be more sensitive and fast than classical technique, allowing the simultaneous detection of I. liriodendri and/or I. macrodidyma and/or "C." pauciseptatum and the identification of unknown inoculum sources for these fungi (eg. callusing-medium and water samples). These studies should be pursued in order to confirm the viability of the inoculum detected.
Descrição: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/4114
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis

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