UTL Repository >
ISA - Instituto Superior de Agronomia >
BISA - Biblioteca do Instituto Superior de Agronomia / Library >
BISA - Dissertações de Mestrado / Master Thesis >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/4110

Title: Utilização da citometria de fluxo multiparamétrica na monitorização da resposta fisiológica da levedura Saccharomyces carlsbergensis em presença de ácido fórmico na produção de bioetanol
Authors: Neves, Elisabete Dias das
Advisor: Silva, Maria Teresa Saraiva Lopes da
Brito, Maria Luísa Castro e
Passarinho, Paula Cristina Varela
Keywords: Saccharomyces carlsbergensis
bioethanol
inhibition
formic acid
multiparameter flow cytometry
Issue Date: 2011
Publisher: ISA/UTL
Citation: Neves, Elisabete Dias - Utilização da citometria de fluxo multiparamétrica na monitorização da resposta fisiológica da levedura Saccharomyces carlsbergensis em presença de ácido fórmico na produção de bioetanol. Lisboa: ISA, 2011
Abstract: The concern with the environmental impacts regarding the use of fossil fuels in the energy sector has triggered the search for new renewable energy sources. The aim of this study was to monitor the physiological response of the yeast Saccharomyces carlsbergensis cells in fermentation assays performed with the addition of formic acid by multi-parameter flow cytometry. The biomass production was more affected by formic acid concentrations higher than 5 g.L-1. Nevertheless, the lowest biomass yield (0,034g.g-1) as well as the lower ethanol yield (35% reduction regarding the assay without inhibitor) were attained with 20g.L-1 of formic acid. The multi-parameter flow cytometry analysis showed that in the presence of 5 g.L-1 of inhibitor during the exponential growth phase there are some stress indicators such as the increase in the number of cells with depolarized membrane and the increase in the number of cells with reactive oxygen species, although the yeast continues to respond in terms of biomass and ethanol productions. The results on ethanol production indicate that the use of this yeast in lignocellulosic hydrolisates fermentation may be interesting due to its resistance to the effect of formic acid when compared to other yeasts like Saccharomyces cerevisae whose inhibition effects of formic acid are registered for concentrations over 4,6 g.L-1 (Larson et al., 2009).
Description: Mestrado em Engenharia de Sistemas Bioenergéticos - Instituto Superior de Agronomia / Instituto Superior Técnico / Faculdade de Ciências. Universidade de Lisboa
URI: http://hdl.handle.net/10400.5/4110
Appears in Collections:BISA - Dissertações de Mestrado / Master Thesis

Files in This Item:

File Description SizeFormat
TeseElisabeteFinal.pdf1.89 MBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 
Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE