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|Title: ||Optimização da produção de açucares por microalgas para a produção de bioetanol|
|Authors: ||Paulo, Vanessa Patrícia Faria|
|Advisor: ||Passarinho, Paula Cristina Varela|
Silva, Maria Luísa Gouveia da
Martins, Maria Luísa Louro
|Issue Date: ||2011|
|Citation: ||Paulo, Vanessa Patrícia Faria - Optimização da produção de açucares por microalgas para a produção de bioetanol. Lisba: ISA, 2011|
|Abstract: ||Nowadays, the world energy crisis and environmental issues are in order. So, the use
of biofuels has gained an extraordinary importance and microalgae seem to be a
promising feedstock, as they do not compete with food either for arable land or for
potable water. Also, they produce several interesting compounds such as sugars and
The aim of this dissertation was the study of sugar production by Spirogyra sp.
regarding the production of bioethanol. So, the first step was the cultivation of this
chlorophyte under continuous lighting and day-night cycles (14 h/10 h), to evaluate the
effect of light regime on the growth and sugar accumulation. Methods for cell rupture
and sugars extraction were also studied and optimized. A yeast screening towards the
production of ethanol from microalgae hidrolysates was the final step of this work.
The highest biomass concentration was attained under day-night cycles (0,34±0,05 vs.
0,29±0,02 gL-1) but the greatest sugar accumulation occurred in the presence of
continuous illumination (27 vs. 24 % g eqglu.g biomass-1). From all the tested methods
for cellular disruption of Spyrogira sp., the most appropriate was acid hydrolysis by
sulfuric acid 2 N in autoclave for 30 min (78 % extracted sugars). S. carlsbergensis
showed to be the most promising microorganism with conversion yields of 82 % for
glucose and mannose. This yeast presented also the ability to use in some extent
arabinose, a pentose found in algae hydrolysates.|
|Description: ||Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia|
|Appears in Collections:||BISA - Dissertações de Mestrado / Master Thesis|
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