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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.5/2369

Title: Identificação de alelos S em diferentes cultivares de pereira e avaliação da sua compatibilidade gametofítica com a 'Rocha'
Other Titles: Identification of S alleles of different pear cultivars of the "Oeste" region and evaluation of their gametophytic compatibility with 'Rocha' pear
Authors: Oliveira, Cristina Moniz
Mota, Mariana
Tavares, Lucélia R.
Keywords: Pyrus communis
S-RNase
gametophytic self-incompatibility
cross pollination
PCR
polinização cruzada
incompatibilidade gametofitica
Issue Date: 2009
Publisher: APH - Associação Portuguesa de Horticultura
Citation: Mota, M.; Tavares, L.; Oliveira, C.M. - Identificação de alelos S em diferentes cultivares de pereira e avaliação da sua compatibilidade gametofítica com a 'Rocha'. In Simpósio Nacional de Fruticultura, 1, Alcobaça, 2006
Series/Report no.: Actas Portuguesas de Horticultura;11
Abstract: In order to select pear cultivar which are genetically compatible with ‘Rocha’ (Sa, Sj), the identification of S alleles sequences of commercial cultivars with bloom periods overlapping (partially or totally) the bloom period of ‘Rocha’ was performed. Partial sequences of the S alleles from the different cultivars were amplified with specific primers, cloned and sequenced. ‘Pêra De Água’ (S22, Sb) ‘Général Leclerc’ (Sl, Sq) and ‘Alexandrine Douillard’ (Sb, Sk) should be totally compatible. It was identified a Sj allele in the cultivars ‘Amêndoa’, ‘Beurré Precoce Morettini’, ‘Clapp’s Rouge’ e ‘Clapp’s Favourite’ and an allele Sa in ‘Beurré Clairgeau’, indicating that these cultivars are semi-compatible with ‘Rocha’. ‘Carapinheira’ (Sb) and ‘Pérola’ (Sk) carry a different allele from the ones of ‘Rocha’ and an amplification pattern completely dissimilar suggesting full compatibility. In ‘Passe Crassane’ (Sr) and ‘Beurré D’Avril’ (S4), a different allele from the S alleles of ‘Rocha’ was identified indicating compatibility, but total or partial compatibility remained unclear, since the amplification pattern does not exclude that the unknown allele might be Sa from ‘Rocha’.
URI: http://hdl.handle.net/10400.5/2369
ISBN: 978-972-8936-04-4
Appears in Collections:DPAA - Comunicações em Actas de Conferências

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