Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/9271
Título: Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk)
Outros títulos: In vitro propagation studies, characterization and valorization of gorse
Autor: Coelho, Maria Teresa Pita Pegado Gonçalves Rodrigues
Orientador: Martins, Margarida Moldão
Gonçalves, José Carlos Dias Duarte
Alves, Vitor Manuel Delgado
Palavras-chave: Pterospartum tridentatum
ethnobotany
micropropagation
aqueous extracts
biological activity
Data de Defesa: 2015
Editora: ISA/UL
Citação: Coelho, M.T.P.P.G.R. - Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk). Lisboa: ISA, 2015, 287 p.
Resumo: The aim of this work was to develop methodologies for micropropagation and extraction of bioactive compounds from both wild gorse (Pterospartum tridentatum L.) and micropropagated gorse explants, as a means of valorisation of this species. Micropropagation was very effective on large scale propagation, having multiplication rates ranging from 1.6 to 43.9; number of shoots ranging from 1.5 to 10.4 and rooting above 96.7%. The extraction yield of aqueous extraction varied from 10.5 to 19.4 and 12.2 to 30.1% (w/w) for wild gorse and micropropagated gorse explants, respectively. Aqueous extracts showed high levels of total phenols, from 203.4 to 369.2 and 104.1 to 121.3 mg gallic acid eq g-1 ms, and FRAP antioxidant activity: 256-856 and 160-260 mmolFe2+ 100g-1 ms; DPPH: 1433-1551 and 1503-1509 mM Trolox 100g-1 ms for wild gorse and micropropagated gorse explants, respectively. Extracts showed no antifungal activity. However, these extracts showed inhibition of Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Chitosan bioactive coatings with gorse extracts improved cherry postharvest preservation ability. The results allow topropose these methodologies to obtain extracts with bioactive properties from micropropagated material, without depleting natural resources
Descrição: Doutoramento em Engenharia Alimentar - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/9271
Aparece nas colecções:BISA - Teses de Doutoramento / Doctoral Thesis

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