Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/9021
Título: Diversity of Iberian nucleopolyhedrovirus wild-type isolates infecting Helicoverpa armigera (Lepidoptera: Noctuidae)
Autor: Figueiredo, Elisabete
Muñoz, Delia
Murillo, Rosa
Mexia, António
Caballero, Primitivo
Palavras-chave: Helicoverpa armigera
tomato fruitworm
Baculovirus
genotypic variants
geographical isolate
pathogenicity
virulence
Data: 2009
Editora: Elsevier
Citação: "Biological Control". ISSN 1049-9644. 50 (2009) 43-49
Resumo: Larvae of the tomato fruitworm, Helicoverpa armigera, were surveyed for nucleopolyhedrovirus (NPV) infection (referred to as HearNPV) in three different locations from the Iberian Peninsula: Olivenza and Toledo in Spain and the Oeste region in Portugal. Twenty HearNPV isolates were obtained from single field-collected larval cadavers. Restriction endonuclease (REN) profiles of the collected isolates with BglII and PstI allowed identification of six different H. armigera single-embedded NPV strains in Spain (referred to as HearSP3, HearSP4, HearSP5, HearSP6, HearSP7, and HearSP8) and two in Portugal (referred to as HearPT1 and HearPT2). No strains were shared by isolates from different geographical regions except HearSP5, which was found in isolates from Olivenza and Toledo. Cluster analysis based on the restriction fragment length polymorphisms of these strains in relation to two previously identified strains from Badajoz (HearSP1) and Cordoba (HearSP2) in Spain, showed no correlation among the strains and their geographical origin. The biological activity of HearSP2, HearSP4, HearSP7, HearSP8, HearPT1, and HearPT2 was compared in terms of pathogenicity (50% lethal concentration, LC50) and virulence (mean time to death). HearPT2 and HearSP7 were significantly more pathogenic than HearSP2, with LC50 values 2.8 and 2.6-fold higher than the latter, respectively, on H. armigera second instars. HearSP4 and HearPT2 killed larvae significantly faster than HearSP8, whereas HearSP2, HearSP7, and HearPT1 showed intermediate mean time to death values
Peer review: yes
URI: http://hdl.handle.net/10400.5/9021
DOI: 10.1016/j.biocontrol.2009.02.005
Versão do Editor: www.elsevier.com.locate/ybcon
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