Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/8517
Título: Produção de lípidos estruturados a partir de óleo de grainha de uva catalisada por lipases não comerciais
Autor: Costa, Carolina Maria Malta da
Orientador: Vicente, Maria Suzana Ferreira Dias
Osório, Natália Maria de Melo
Palavras-chave: structured lipids
low calorie lipids
human milk fat substitutes
grapeseed oil
lipaze from Rhyzopus oryzae
Carica papaya latex
Data de Defesa: 2015
Editora: ISA/UL
Citação: Costa, C.M.M. - Produção de lípidos estruturados a partir de óleo de grainha de uva catalisada por lipases não comerciais. Lisboa: ISA, 2015, 77 p.
Resumo: The aim of this study was the production of structured lipids, namely, human milk fat substitutes (HMFS), and low calorie triacylglycerols (TAG) of the MLM type, using grapeseed oil (GO). The acidolysis reactions were performed in batch, in solvent-free systems and catalyzed by the heterologous lipase from Rhizopus oryzae (rROL) immobilized in Amberlite™ IRA 96 or by Carica papaya lipase (CPL) self-immobilized in papaya latex. In the HMFS production, at 60 °C, tripalmitin was used as source of TAG, and GO as source of polyunsaturated fatty acids, especially C18:2. The obtained yield in new TAG was 10.4 % (w/w) with rROL. The CPL showed to be enable to catalyze this reaction. The MLM production occurred at 40 °C, by acydolisis between GO and caprylic (C8:0) or capric (C10:0) acids, and the yields in new TAG varied between 36.2 and 66.8 % (w/w). Thus, MLM production reactions were carried out at 30, 40 and 50 °C, during 48h, and the major productions of new TAG were achieved at 40 °C, with both biocatalysts. Operational stability tests, at 40°C, were carried out, and the half-life times obtained were 166 and 91h for rROL and 96 and 81h for CPL, respectively, in GO acidolysis with C8:0 and C10:0.
Descrição: Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/8517
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis

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