Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/8110
Título: Chromosome and DNA methylation dynamics during meiosis in autotetraploid Arabidopsis arenosa
Autor: Carvalho, Ana
Delgado, Margarida
Barão, Augusta
Frescatada, Márcia
Ribeiro, Edna
Pikaard, Craig S.
Viegas, Wanda
Neves, Nuno
Palavras-chave: meiosis
autopolyploid
DNA methylation
Arabidopsis arenosa
Data: 2010
Editora: Springer
Citação: "Sexual Plant Reproduction". ISSN 0934-0882. 23 (2010) 29-37
Resumo: Variation in chromosome number due to polyploidy can seriously compromise meiotic stability. In autopolyploids, the presence of more than two homologous chromosomes may result in complex pairing patterns and subsequent anomalous chromosome segregation. In this context, chromocenter, centromeric, telomeric and ribosomal DNA locus topology and DNA methylation patterns were investigated in the natural autotetraploid, Arabidopsis arenosa. The data show that homologous chromosome recognition and association initiates at telomeric domains in premeiotic interphase, followed by quadrivalent pairing of ribosomal 45S RNA gene loci (known as NORs) at leptotene. On the other hand, centromeric regions at early leptotene show pairwise associations rather than associations in fours. These pairwise associations are maintained throughout prophase I, and therefore likely to be related to the diploid-like behavior of A. arenosa chromosomes at metaphase I, where only bivalents are observed. In anthers, both cells at somatic interphase as well as at premeiotic interphase show 5-methylcytosine (5-mC) dispersed throughout the nucleus, contrasting with a preferential co-localization with chromocenters observed in vegetative nuclei. These results show for the first time that nuclear distribution patterns of 5-mC are simultaneously reshuffled in meiocytes and anther somatic cells. During prophase I, 5-mC is detected in extended chromatin fibers and chromocenters but interestingly is excluded from the NORs what correlates with the pairing pattern
Peer review: yes
URI: http://hdl.handle.net/10400.5/8110
DOI: 10.1007/s00497-009-0115-2
Aparece nas colecções:CBAA - Artigos de Revista

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