Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/7324
Título: Listeria monocytogenes interstrain comparative exoproteome analysis at different temperatures
Outros títulos: Análise comparativa de exoproteomas de Listeria monocytogenes em função da estirpe e da temperatura
Autor: Cunha, Paula Cristina Branco Cabrita
Orientador: Brito, Maria Luísa de Castro
Ferreira, Ricardo Boavida
Trigo, Maria João Almeida Pessoa
Palavras-chave: public health sciences
Listeria monocytogenes
exoproteins
qPCR
temperature
Data de Defesa: 2014
Editora: ISA/UL
Citação: Cunha, P.C.B.C. - Listeria monocytogenes interstrain comparative exoproteome analysis at different temperatures. Lisboa: ISA, 2014, 173 p.
Resumo: The present work focused on exoproteomic and transcriptomic analyses of four genetic distinct Listeria monocytogenes strains including one persistent strain. The results showed major differences in the exoproteomes of the strains grown in minimal medium at 37 °C, 20_°C and 11 °C. When grown at 37 °C, the virulence of the strains could be correlated with predicted from the levels of listeriolysin O and internalin C. At 11 °C and 20 °C, the main finding was the lack of flagellin and of oligopeptide permease protein A in the persistent strain. Transcriptional levels of nine cold stress related genes in two of these strains, grown at 11 °C, indicated that the persistent strain presented higher transcript levels of dtpT and sigB, and lower transcript levels of flaA, oppA, lmo1722 and lmo0866. The swarming motility of a group of 23 persistent and non-persistent strains, at the same temperature, indicated that motility per se should not be considered determinant for persistence. An integrated exoproteomic, transcriptomic and phenotypic analysis on a broader set of persistent and non-persistent strains may improve the knowledge on L. monocytogenes regulatory networks towards prevention and control of this foodborne pathogen in food and food related environments
Descrição: Doutoramento em Engenharia Alimentar - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/7324
Aparece nas colecções:BISA - Teses de Doutoramento / Doctoral Thesis

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