Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/7176
Título: Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
Autor: Ferreira, A. M.
Araújo, S. S.
Sales-Baptista, E.
Almeida, A. M.
Palavras-chave: Bitter taste
Taste receptors
T2R
Sheep
Ingestive behavior
Data: Abr-2013
Editora: The Animal Consortium
Citação: Ferreira, A.M., Araújo, S.S., Sales-Baptista, E., Almeida, A.M. (2013). Identification of novel genes for bitter taste receptors in sheep (Ovis aries). Animal, 7(4), 547-554. doi: 10.1017/S1751731112002030
Resumo: Genetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates in sheep by comparison with the described T2R in cattle and using recently available ovine genome. In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5. The in silico predicted genes were then confirmed by PCR and DNA sequencing. The sequencing results showed a 99% to 100% similarity with the in silico predicted sequence. Moreover, we address the chromosomal distribution and compare, in homology and phylogenetic terms, the obtained genes with the known T2R in human, mouse, dog, cattle, horse and pig. The eight novel genes identified map either to ovine chromosome 3 or 4. The phylogenetic data suggest a clustering by receptor type rather than by species for some of the receptors. From the species analyzed, we observed a clear proximity between the two ruminant species, sheep and cattle, in contrast with lower similarities obtained for the comparison of sheep with other mammals. Although further studies are needed to identify the complete T2R repertoire in domestic sheep, our data represent a first step for genetic studies on this field.
Descrição: Articles in International Journals
Peer review: yes
URI: http://hdl.handle.net/10400.5/7176
DOI: 10.1017/S1751731112002030
ISSN: 1751-732X (online)
Versão do Editor: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8844054&fileId=S1751731112002030
Aparece nas colecções:CIISA - Artigos em revistas internacionais



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