Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/6676
Título: Estudo genético e funcional de genes HESP envolvidos na patogenicidade de Hemileia vastatrix (Ferrugem alaranjada do cafeeiro)
Autor: Gonçalves, Ana Cristina Fernandes dos Santos
Orientador: Tavares, Sílvia Andreia Godinho Branquinho
Oliveira, Maria Helena Mendes da Costa Ferreira Correia de
Palavras-chave: avirulence genes
coffee leaf rust
efectores
Hemileia vastatrix
RT-qPCR
production of recombinant proteins
Data de Defesa: 2013
Editora: ISA
Citação: Gonçalves, A.C.F.S. - Estudo genético e funcional de genes HESP envolvidos na patogenicidade de Hemileia vastatrix (Ferrugem alaranjada do cafeeiro). Lisboa: ISA, 2013, 92 p.
Resumo: Coffee leaf rust, caused by Hemileia vastratrix, is a disease with a major impact on the economy of coffee production. The H. vastatrix – coffee interaction follows the gene for gene theory, where a resistance (R) protein located in the host cytoplasm recognizes an avirulence (Avr) protein secreted by the fungal haustoria, triggering the hypersensitive response. The major goal of this study was the identification of H. vastatrix candidate effectors, namely Avr genes. To this end, Expressed Sequence Tags (ESTs) from 454-pyrosequencing, generated during previous projects of the Centro de Investigação de Ferrugens do Cafeeiro (Oeiras), were used. The MEME bioinformatic algorithm enabled the identification of four sequences, Hv02363, Hv04351, Hv04352 and Hv04556, that shared three conserved protein motifs, in which two of them were constituted by the amino acid cysteine. The gene expression in coffee leaves (Coffea arabica H147/1), inoculated with H. vastatrix (race XIV), was quantified by qPCR at 7 day post-inoculation and revealed that all genes had a strong induction of expression, which was coincident with the increase in haustorium formation. In order to produce specific antibodies for performing immunolocalization, a successful cloning and purification of the recombinant protein of the gene Hv02363 was conducted. Overall, the data reported here represent first steps towards a better molecular understanding of rust-coffee interaction, targeting the selection of new coffee resistant varieties.
Descrição: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/6676
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis



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