Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/6487
Título: Identificação de marcadores SSR e de SNPs em medronheiro (Arbutus unedo L.) por sequenciação massiva paralela
Autor: Fazenda, Pedro Miguel Jesus
Orientador: Leitão, José Manuel Teixeira
Oliveira, Cristina Maria Moniz Simões
Palavras-chave: Arbutus unedo
CAPS
molecular markers
next-generation sequencing
nuclear DNA extraction
SSRs
Data de Defesa: 2013
Editora: ISA
Citação: Fazenda, P.M.J. - Identificação de marcadores SSR e de SNPs em medronheiro (Arbutus unedo L.) por sequenciação massiva paralela. Lisboa: ISA, 2013, 42 p.
Resumo: The strawberry tree (Arbutus unedo L.) is native to the Mediterranean region. The use of molecular markers in this species has been limited to the use of RAPDs, ISSRs as well to the cross-amplification of SSRs from other Ericaceae. In this work, we developed a protocol for extracting nuclear DNA from the strawberry tree and performed partial next-generation sequencing of the Arbutus unedo L. genome using the "Ion Torrent" (Life Technologies) platform. The next-generation sequencing resulted in 198,856 sequences ("raw data") with an average size of 123 bp, which were uploaded to the NCBI database "Sequence Read Archive" (SRA) with the accession number: SRX341237. Data analysis led to the identification of 1085 microsatellite-containing sequences, which were also uploaded with accession numbers: from KF023636 to KF024720 to the NCBI databases. Primers were designed for 18 microsatellite loci of which only three have proved to be polymorphic in a panel of 16 samples. Based on identified 25 SNPs one CAPS marker was developed, which despite being heterozygous revealed to be monomorphic among the 16 analyzed samples.
Descrição: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/6487
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis

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