Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/6107
Título: An integrated BAC approach to the genomics of cork formation
Autor: Pinto, Maria Joana Amado Teixeira
Orientador: Paiva, Jorge Pinto
Cecílio, Leonor Morais
Palavras-chave: cork
suberin
BAC library
BESs
FISH
quantitative real time PCR
Data de Defesa: 2013
Editora: ISA
Citação: Pinto, M.J.A.T. - An integrated BAC approach to the genomics of cork formation. Lisboa: ISA, 2013, 129 p.
Resumo: Cork is produced by Quercus suber, an evergreen oak with major economic and environmental importance in Portugal. Suberin is the main component of cork but little is known about the molecular processes underlying suberin biosynthesis. We screened a Quercus suber bacterial artificial chromosome (BAC) library for the presence of relevant genes for suberin biosynthesis through high-density BAC filters hybridizations and we validated hybridization positive clones by Colony PCR. We analyzed BAC-end sequences (BESs) to characterize the structure and composition of the cork oak genome. And we hybridized BAC DNA with mitotic cork oak chromosomes to obtain a cytogenetic map of Q. suber. Finally, we analyzed expression profiles of candidate genes of cork samples from cork oaks with different cork qualities, using quantitative real-time PCR. We identified several genes involved in suberin biosynthesis pathways. BESs analysis showed high identity with coding regions and suggest the existence of conserved regions within the Fagacea family. We found strong evidence that the genes Cyp 86A1 and GDSL involved in lipid suberin biosynthesis pathway are closely located in the genome. However, we did not succeed in mapping genes of interest in the cork oak genome and we did not find any relation between the genes relative expression and cork quality
Descrição: Mestrado em Biologia Funcional - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/6107
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis

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