Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/5802
Título: Membrane tension regulates water transport in yeast
Autor: Soveral, Graça
Madeira, Ana
Loureiro-Dias, Maria C.
Moura, Teresa F.
Palavras-chave: yeast
Saccharomyces cerevisiae
water transport regulation
aquaporin
membrane tension
Data: 2008
Editora: Elsevier
Citação: "Biochimica et Biophysica Acta". ISSN 0005-2736. 1778 (2008) 2573-2579
Resumo: Evidence that membrane surface tension regulates water fluxes in intact cells of a Saccharomyces cerevisiae strain overexpressing aquaporin AQY1 was obtained by assessing the osmotic water transport parameters in cells equilibrated in different osmolarities. The osmotic water permeability coefficients (Pf) obtained for yeast cells overexpressing AQY1 incubated in low osmolarity buffers were similar to those obtained for a double mutant aqy1aqy2 and approximately three times lower (with higher activation energy, Ea) than values obtained for cells incubated in higher osmolarities (with lower Ea). Moreover, the initial inner volumes attained a maximum value for cells equilibrated in lower osmolarities (below 0.75 M) suggesting a preswollen state with the membrane under tension, independent of aquaporin expression. In this situation, the impairment of water channel activity suggested by lower Pf and higher Ea could probably be the first available volume regulatory tool that, in cooperation with other osmosensitive solute transporters, aims to maintain cell volume. The results presented point to the regulation of yeast water channels by membrane tension, as previously described in other cell systems
Peer review: yes
URI: http://hdl.handle.net/10400.5/5802
ISSN: 0005-2736
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