Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/3864
Título: Molecular responses of Coffea spp. tolerance to low non-freezing temperatures
Autor: Santos, Paula Cristina Bento Batista dos
Orientador: Ribeiro, Ana Isabel Faria
Chaves, Maria Manuela
Palavras-chave: Coffea
antioxidative molecules
cold
photosynthesis
chloroplast membrane lipids
gene expression
Data de Defesa: 2010
Editora: ISA/UTL
Citação: Santos, Paula Cristina Bento Batista dos - Molecular responses of Coffea spp. tolerance to low non-freezing temperatures. Lisboa: ISA, 2010
Resumo: Low positive temperatures are of up most importance in tropical plant species, namely in Coffea spp., disturbing plant growth and metabolism, with impact on photosynthesis and yield. An integrated biochemical and molecular approach was used to analyze the cold impact and tolerance ability on photosynthesis of coffee plants, linked to modifications of the status of the antioxidative system, and chloroplast membrane lipids. Five coffee genotypes with contrasting cold sensitivity were used: Coffea canephora cv. Apoatã, C. arabica cv. Catuaí, C. dewevrei, Icatu (C. arabica x C. canephora) and Piatã (C. dewevrei x C. arabica). Determinations were performed along a slow cold imposition (to allow acclimation) from 25/20 ºC (day/night) down to 13/8 ºC, after exposure to 4o C (chilling) and in the rewarming period thereafter. Cold exposure strongly affected net photosynthesis in all genotypes, although stomatal limitations were not detected. Icatu revealed the lowest leaf loss, higher reinforcement of the antioxidative system, qualitative changes in chloroplast membrane lipids and regulation of some key genes. Altogether these responses indicate a better ability of this genotype to cope with low positive temperatures
Descrição: Doutoramento em Biologia - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/3864
Aparece nas colecções:BISA - Teses de Doutoramento / Doctoral Thesis

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