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Title: Collection of Lupinus angustifolius L. germplasm and characterisation of morphological and molecular diversity
Author: Talhinhas, Pedro
Leitão, José
Martins, João Neves
Keywords: AFLP
genetic diversity
germplasm collection
Lupinus angustifolius
Issue Date: 2006
Publisher: Springer
Citation: "Genetic Resources and Crop Evolution".ISSN 1573-5109. 53 (2006) 563-578
Abstract: Lupinus angustifolius L. is a Mediterranean species, domesticated in the 20th century, representing an important grain legume crop in Australia and other countries. This work is focused on the collection of wild germplasm and on the characterisation of morphological and molecular diversity of germplasm accessions. It reports the collection of 81 wild L. angustifolius accessions from the South and Centre of Portugal, available at the ‘Instituto Superior de Agronomia Gene Bank’, with subsequent morphological and molecular characterisation of a selection of these and other accessions. A multivariate analysis of morphological traits on 88 L. angustifolius accessions (including 59 wild Portuguese accessions, 15 cultivars and 14 breeding lines) showed a cline of variation on wild germplasm, with plants from Southern Portugal characterised by earlier flowering, higher vegetative development and larger seeds. AFLP and ISSR molecular markers grouped modern cultivars as sub-clusters within the wider diversity of wild germplasm, revealing the narrow pool of genetic diversity on which domesticated accessions are based. The importance of preserving, characterising and using wild genetic resources for L. angustifolius crop improvement is outlined by the results obtained.
Peer review: yes
ISSN: 1573-5109
Appears in Collections:DBEB - Artigos de Revistas

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