Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/2851
Título: Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
Autor: Pereira, H. Sofia
Barão, Augusta
Caperta, A.
Rocha, João
Viegas, Wanda
Delgado, Margarida
Palavras-chave: E3900 rye B repeat
developmentally regulated transcription
chromosome segregation
Data: 2009
Editora: Oxford University Press
Citação: "Molecular Biology and Evolution". 26:8 (2009) 1683-1697
Resumo: Two sequence families, E3900 and D1100, are amplified on the subtelomeric domain of the long arm of rye B chromosomes, the region that controls its drive mechanism. In this work, polymerase chain reaction (PCR) with a number of primers spanning E3900 shows that the organization and nucleotide sequence of E3900-related portions are present and highly conserved on rye A chromosomes as well as in other cereals. Quantitative Real-Time PCR estimates two E3900 sequences to be represented in 100–150 copies on Bs and at least as single copies on As. A novel E3900- related sequence, with a deletion that results in a frameshift and subsequently an open reading frame with putative DNA binding motifs, is identified. Expression analysis of E3900 indicates identical transcription levels in leaves from plants with and without Bs, showing that the expression of these sequences must be silenced on Bs and tightly regulated on As in leaves. In contrast, E3900 transcription is upregulated during meiosis exclusively in plants with Bs, maintaining a high level of transcription in the gametophyte. Interestingly, Bs not only influence their own chromatid segregation but also that of the regular chromosome complement of both rye and wheat. There is a drastic increase in frequency of disrupted metaphase and anaphase cells in the first mitosis of pollen grains carrying Bs, which appears to be due to anomalous adherences between sister chromatids. Taken together, this work provides insight into how E3900 sequences are potentially associated with important evolutionary mechanisms involved in basic cellular processes.
Descrição: Research Article
URI: http://hdl.handle.net/10400.5/2851
Aparece nas colecções:CBAA - Artigos de Revista

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REP-7-2009 Pereira et al. MBE Rye Bs Disclose Ancestral Sequences in Cereal Genomes with a Potential Role in.pdf866,6 kBAdobe PDFVer/Abrir


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