Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/2844
Título: Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
Autor: Caperta, A.
Neves, Nuno
Morais-Cecílio, L.
Malhó, Rui
Viegas, Wanda
Palavras-chave: homologous gene expression
rDNA topology
chromosome structure
telocentric chromosomes
rye
Data: 2002
Editora: The Company of Biologists
Citação: "Journal of Cell Science". 115 (2002) 2839-2846
Resumo: The standard rye cultivar ‘Imperial’ and a structural variant carrying an intact 1R chromosome and two telocentric 1R chromosomes (short and long arms) were used to investigate expression patterns of homologous rDNA loci, and the influence of chromosome structural change on their interphase organisation and relative disposition. Sequential silver staining and in situ hybridization with the rDNA probe pTa71, established a correspondence between the expression and organization patterns of rDNA domains in metaphase and interphase cells. In most cells of the cultivar Imperial, nucleolar organizer region (NOR) silver staining on metaphase chromosomes with equivalent numbers of rDNA genes revealed a size heteromorphism between homologousrDNA loci, resulting from their differential expression. NOR heteromorphism in the structural variant line was significantly reduced. The preferential activity of one NOR over its homologue was found to be random within cells and independent of parental origin. Nucleotypic modifications mediated by changes in the 1R chromosome structure include increased proximity between homologous rDNA loci in interphase, and an increase in the frequency of cells with intra-nucleolar ribosomal condensed chromatin. These results seem to indicate a ‘sequence recognition’ process for the regulation of homologous loci.
Descrição: Research Article
URI: http://hdl.handle.net/10400.5/2844
Aparece nas colecções:CBAA - Artigos de Revista

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