Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.5/2488
Título: Respostas celulares e moleculares em interacções hospedeiras e não hospedeiras cafeeiro-ferrugem (Hemileia vastatrix e Uromyces vignae)
Outros títulos: Cellular and molecular responses in host and non-host coffee-rust interactions (Hemileia vastatrix ans Uromyces vignae)
Autor: Diniz, Inês Isabel Plácido dos Santos
Orientador: Silva, Maria do Céu Machado Lavado
Oliveira, Maria Helena Mendes da Costa Ferreira Correia de
Palavras-chave: coffee
coffee leaf rust
host resistance
non-host resistance
hypersensitive reaction
gene expression
cafeeiro
ferrugem alaranjada
resistência hospedeira
resistência não-hospedeira
reacção de hipersensibilidade
expressão génica
Data de Defesa: 2010
Resumo: Cellular and molecular characterization of resistance to H. vastatrix in coffee lines HDT 832/2 and Sarchimor, was conducted using the non-host resistance model coffee-Uromyces vignae (cow pea rust) for comparison. In both lines, H. vastatrix ceased growth more frequently at the penetration hypha stage, forming few haustoria, inducing hypersensitive reaction (HR), phenol accumulation and haustorium encasement with calose. U. vignae growth was more restricted in HDT 832/2, but failed to form haustoria in both lines, while inducing HR and phenol accumulation. The expression of genes involved in mechanisms of recognition, signaling and defence (rlk, wrky1, pal, chs, 13-lox, gt, pr1b and pr10) was monitored by real time PCR. Both in the host and non-host interactions the expression profile of rlk and wrky1 suggests that the recognition of the fungus and signaling occur during the formation of appressoria over stomata and the differentiation of the first post-penetration infection stages. The first activation peak of 13-lox coincided with the onset of cell death detection. pr1b exhibited high expression levels in the host interaction, being poorly activated in the non-host interaction. These results suggest that both JA and SA pathways may coexist, although SA pathway seams to prevail in the host interaction.
Descrição: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
URI: http://hdl.handle.net/10400.5/2488
Aparece nas colecções:BISA - Dissertações de Mestrado / Master Thesis

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